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Enhancing plasma peptide MALDI-TOF-MS profiling by mesoporous silica assisted crystallization |
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| Authors: | Rosa Terracciano Francesca Casadonte Patrizio Candeloro Andrea Urbani |
| Affiliation: | a Department of Experimental and Clinical Medicine, “Magna Graecia” University of Catanzaro, 88100 Catanzaro, Italy b Department of Chemical Engineering and Materials, University of Calabria, 87036 Cosenza, Italy c University of Rome “Tor Vergata”, Department of Internal Medicine and S. Lucia Foundation - IRCCS, Rome, Italy |
| Abstract: | Promising profiling techniques based on new material/solid phase extraction for capturing “molecular signatures” from body fluids are being coupled to MALDI-TOF-MS. Sample preparation significantly influences spectrum quality in this ionization method. Mesoporous silica beads (MSB), by the means of nano-sized porous channels with high surface area, enable harvesting of peptides from plasma and serum excluding large size proteins. We have investigated the morphology of a sample slurry, developed as a new tool for plasma peptides enrichment based on mesoporous materials. Our study highlights a correlation between crystals morphology and enhanced performances in MALDI-TOF-MS analysis. This is the first report which correlates the increase in signal intensity with crystal formation in samples preparations which make use of various kinds of slurries for the analysis of samples clinically relevant like human plasma. |
| Keywords: | Clinical proteomics Biomarkers discovery MALDI-TOF-MS Mesoporous materials Human plasma |
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