Detection of s-triazine pesticides in natural waters by modified large-volume direct injection HPLC |
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Authors: | David J Beale Sarit L Kaserzon Felicity A Roddick Peter D Carpenter |
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Institution: | a School of Applied Sciences, RMIT University, GPO Box 2476, Melbourne 3001, Australia b School of Civil, Chemical and Environmental Engineering, RMIT University, GPO Box 2476, Melbourne 3001, Australia c Water Quality Research Australia (Formally the CRC for Water Quality and Treatment), Private Mail Bag 3, Salisbury, South Australia 5108, Australia |
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Abstract: | There is a need for simple and inexpensive methods to quantify potentially harmful persistent pesticides often found in our water-ways and water distribution systems. This paper presents a simple, relatively inexpensive method for the detection of a group of commonly used pesticides (atrazine, simazine and hexazinone) in natural waters using large-volume direct injection high performance liquid chromatography (HPLC) utilizing a monolithic column and a single wavelength ultraviolet-visible light (UV-vis) detector. The best results for this system were obtained with a mobile phase made up of acetonitrile and water in a 30:70 ratio, a flow rate of 2.0 mL min−1, and a detector wavelength of 230 nm. Using this method, we achieved retention times of less than three minutes, and detection limits of 5.7 μg L−1 for atrazine, 4.7 μg L−1 for simazine and 4.0 μg L−1 for hexazinone. The performance of this method was validated with an inter-laboratory trial against a National Association of Testing Authorities (NATA) accredited liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS) method commonly used in commercial laboratories. |
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Keywords: | Atrazine Direct injection HPLC Large-volume injection Monolithic column Hexazinone Natural organic matter Natural waters Simazine |
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