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Tin oxide nanoparticles-polymer modified single-use sensors for electrochemical monitoring of label-free DNA hybridization |
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| Authors: | Mihrican Muti Filiz Kuralay Arzum Erdem Serdar Abaci Ali Sina? |
| Affiliation: | a Ege University, Faculty of Pharmacy, Analytical Chemistry Dept., Bornova, 35100 Izmir, Turkey b Adnan Menderes University, Faculty of Science, Chemistry Department, 09010 Ayd?n, Turkey c Hacettepe University, Faculty of Science, Department of Chemistry, Beytepe, 06532 Ankara, Turkey d Ankara University, Faculty of Science, Department of Chemistry, Be?evler, 06100 Ankara, Turkey |
| Abstract: | In this study, SnO2 nanoparticles (SNPs)-poly(vinylferrocenium) (PVF+) modified single-use graphite electrodes were developed for electrochemical monitoring of DNA hybridization. The surfaces of polymer modified and polymer-SNP modified pencil graphite electrodes (PGEs) were firstly characterized by using SEM analysis. The electrochemical behaviours of these electrodes were also investigated using the differential pulse voltammetry (DPV) and electrochemical impedance spectroscopy (EIS) techniques. The polymer-SNP modified PGEs were then tested for the electrochemical sensing of DNA based on the changes at the guanine oxidation signals. Experimental parameters, such as; different modifications in DNA oligonucleotides, DNA probe concentrations were examined to obtain more sensitive and selective electrochemical signals for nucleic acid hybridization. After optimization studies, DNA hybridization was investigated in the case of complementary of hepatitis B virus (HBV) probe, mismatch (MM), and noncomplementary (NC) sequences. |
| Keywords: | Tin oxide nanoparticle Polymer Poly(vinylferrocenium) DNA sensor Guanine Pencil graphite electrode |
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