Molecular Networking and Cultivation Profiling Reveals Diverse Natural Product Classes from an Australian Soil-Derived Fungus Aspergillus sp. CMB-MRF324 |
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Authors: | Taizong Wu Angela A. Salim Paul V. Bernhardt Robert J. Capon |
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Affiliation: | 1.Institute for Molecular Bioscience, The University of Queensland, Brisbane, QLD 4072, Australia;2.School of Chemistry and Molecular Bioscience, The University of Queensland, Brisbane, QLD 4072, Australia |
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Abstract: | This study showcases the application of an integrated workflow of molecular networking chemical profiling (GNPS), together with miniaturized microbioreactor cultivation profiling (MATRIX) to successfully detect, dereplicate, prioritize, optimize the production, isolate, characterize, and identify a diverse selection of new chemically labile natural products from the Queensland sheep pasture soil-derived fungus Aspergillus sp. CMB-MRF324. More specifically, we report the new tryptamine enamino tripeptide aspergillamides E–F (7–8), dihydroquinoline-2-one aflaquinolones H–I (11–12), and prenylated phenylbutyrolactone aspulvinone Y (14), along with an array of known co-metabolites, including asterriquinones SU5228 (9) and CT5 (10), terrecyclic acid A (13), and aspulvinones N-CR (15), B (16), D (17), and H (18). Structure elucidation was achieved by a combination of detailed spectroscopic and chemical analysis, biosynthetic considerations, and in the case of 11, an X-ray crystallographic analysis. |
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Keywords: | Aspergillus Australian soil-derived fungus aspergillamide asterriquinone aflaquinolone aspulvin aspulvinone GNPS molecular networking MATRIX cultivation |
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