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枯草杆菌原生质体再生前后细胞壁的变化及其对酶渗透性的影响
引用本文:王忠彦,孙启玲,胡永松,林巧.枯草杆菌原生质体再生前后细胞壁的变化及其对酶渗透性的影响[J].四川大学学报(自然科学版),1989(3).
作者姓名:王忠彦  孙启玲  胡永松  林巧
作者单位:四川大学生物工程系 (王忠彦,孙启玲,胡永松),四川大学生物工程系(林巧)
摘    要:报道了枯草芽孢杆菌Bacillus subtilis BF—7658原生质体的制备、再生及其传代后的细胞的电镜观察;酶产量的变化;初步证明经原生质体再生所得到的α——淀粉酶高产菌株,同其细胞壁再生过程及细胞壁的渗透性有着密切关系.

关 键 词:枯草芽抱杆菌  原生质体  细胞壁  淀粉酶

STUDIES ON THE YIELD OF a-AMYLASE AND THE CHANGE OF CELL WALL OF CELLS REGENERATED FROM BACILLUS SUBTILIS PROTOPLAST
Wang Zhongyan Sun Qiling Hu Yongsong Lin Qiao.STUDIES ON THE YIELD OF a-AMYLASE AND THE CHANGE OF CELL WALL OF CELLS REGENERATED FROM BACILLUS SUBTILIS PROTOPLAST[J].Journal of Sichuan University (Natural Science Edition),1989(3).
Authors:Wang Zhongyan Sun Qiling Hu Yongsong Lin Qiao
Institution:Department of Biotechnology
Abstract:In the experiment the regenerated cells from Bacillus subtilis BF-7658 protoplast wete inoculated filial generation by filial generation and cells of filial generation were reviewed by scanning electron microscope and also a-amylase activity in medium was determined.The results showed that the regenerating strains with high yielded a- amylase were relative to the incomptete cell wall regenrated.
Keywords:bacillus subtilis  protoplast  cell wall  a-amylase  
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