血液灌流吸附清除中分子物质的实验研究

我们从所研制的系列树脂中筛选出对中分子物质(MMS)吸附作用较好的5种吸附树脂,并将其中HP-A_3和HP-A_9用于尿毒症患者血浆体外模拟灌流和急性肾衰大鼠血液灌流。应用MMS总量测定法和Sephadex G25凝胶层析法研究树脂对MMS的吸附清除作用。结果表明,这两种树脂体内、外清除MMS作用显著,HP-A_9树脂体内清除效果更好,其血液相容性也更为理想,有希望应用于临床治疗某些MMS堆积的疾病。

Experimental Study on Removal of Middle Molecular Substances by Adsorbent Hemoperfusion

A Series of resins were screened for the adsorption of middle molecular substances(MMS).Five of them were chosen for their effective adsorption of MMS. HP-A3 and HP-A9 resins were used for perfusion of uremic plasma and hemoperfusion of rats with acute renal failure(ARF). We studied the removal of MMS by adsorbent resins with measurement of the total amount of MMS and with the help of Sephadex G-25 chromatography of the plasma. The results showed that MMS could be removed dramatically by HP-A3 and HP-A9 resins. The total amount of MMS decreased by 49.6% and 36.5% respectively while the peak height unit of MMS decreased by 73.3% and 75.5% respectively by perfusion for 90 minutes of the uremic plasma. The rats with ARF were hemoperfused. HP-A3 resin could remove 11.7% of the total amount of MMS by hemoperfusion for 60 minutes and HP-A9 resin could remove 20.3% and 31.7% of them for 60 and 90 minutes respectively HP-A9 resin was more effective in the adsorption of MMS in vivo, and was of better blood compatibility.HP-A9 resin has good expectation for the treatment of some MMS related diseases.