A simple and fast LC–MS/MS method for the simultaneous determination of tenofovir alafenamide and tenofovir in human plasma |
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| Authors: | Xiaoping Qian Qi Chen Yunhui Chen Shiliang Ji Ying Wang Ye Sun Huixin Qi Kan Zhong Jinfang Jiang Xin Chen Lifeng Huang Yong Yang |
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| Institution: | 1. The Affiliated Suzhou Science and Technology Town Hospital of Nanjing Medical University, Suzhou, China;2. Suzhou Haike Medical Technology Co. Ltd., Suzhou, China;3. Suzhou Biyi Biotechnology Co. Ltd., Suzhou, China |
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| Abstract: | A simple and fast liquid chromatography–tandem mass spectrometry method was established and validated for the simultaneous determination of tenofovir alafenamide (TAF) and tenofovir (TNF) in human plasma. A simple protein precipitation procedure was employed to extract analytes from plasma. Chromatographic separation was performed on an Eclipse Plus C18 column utilizing a fast gradient elution starting with 2% of 2 mM ammonium acetate–formic acid (100/0.1, v/v) followed by increasing the percentage of acetonitrile. Detection was performed on a tandem mass spectrometer equipped with an electrospray ionization source operated in the positive ionization mode, using the transitions m/z 477.2 → m/z 346.1 for TAF and m/z 288.1 → m/z 176.1 for TNF. TAF-d5 and TNF-d7 were used as the internal standard of TAF and TNF, respectively. The method was validated in the concentration ranges 1.25–500 ng/mlfor TAF and 0.300–15.0 ng/ml for TNF with acceptable accuracy and precision. |
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| Keywords: | determination LC–MS/MS metabolite tenofovir tenofovir alafenamide |
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