(1) E. Merck, Reagent Division, R+D Chromatography, D-6100 Darmstadt, FRG;(2) Pharmaceutical Department, Sandoz Ltd., Basle, Switzerland;(3) Institute of Analytical Chemistry, University of Vienna, Vienna, Austria
Abstract:
Summary The criteria for the optimization of preparative chromatography are sample throughput and product purity. It is shown for column liquid chromatography that the throughput has been maximized for a given purity by column overloading and appropriate fractionation. The size and position of the fraction must be determined by chemical analysis, since overlapping peaks under overloading conditions are usually distorted compared to the peaks of the same amounts of pure compounds. With overloading of the column larger particles can be used as column packings without reduction of the separation effect.