A mitochondrial-targeted two-photon probe for zinc ion |
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| Authors: | Masanta Goutam Lim Chang Su Kim Hyung Joong Han Ji Hee Kim Hwan Myung Cho Bong Rae |
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| Institution: | Molecular Science and Technology Research Center, Ajou University, Suwon 443-749, Korea. |
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| Abstract: | We report a two-photon probe (SZn-Mito) for mitochondrial zinc ions (Zn2+]m). This probe shows a 7-fold enhancement of two-photon-excited fluorescence in response to Zn2+ with a dissociation constant (Kd(TP)) of 3.1 ± 0.1 nM and pH insensitivity in the biologically relevant range, allowing the detection of Zn2+]m in a rat hippocampal slice at a depth of 100?200 μm without interference from other metal ions through the use of two-photon microscopy. |
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