| 大鼠脑缺血再灌注后运动皮质细胞凋亡的体视学观察 |
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| 引用本文： | 辜清,万勇,吴娣,姚立希,杨琳.大鼠脑缺血再灌注后运动皮质细胞凋亡的体视学观察[J].南昌大学学报(理科版),2009,33(1):1. |
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| 作者姓名： | 辜清 万勇 吴娣 姚立希 杨琳 |
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| 作者单位： | 南昌大学生命科学学院； |
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| 摘 要： | 用体视学方法分析大鼠脑缺血再灌注后大脑运动皮质细胞的凋亡情况。随机将24只SD大鼠分为缺血再灌注后6、12、24 h和对照组。采用结扎大鼠左侧颈总动脉和夹闭右侧颈总动脉15m in后放开的方法制备大鼠脑缺血再灌注模型。应用Hoechst33258荧光染色及免疫组化Bax蛋白表达来检测细
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| 关 键 词： | 体视学 脑缺血 再灌注 Bax 凋亡 Hoechst33258 |
Observation on Stereology of Cell Apoptosis in Cerebral Parietal Cortex after Cerebral Ischemia Reperfusion in the Rats |
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| GU Qing,WAN Yong,WU Di,YAO Li-xi,YANG Li.Observation on Stereology of Cell Apoptosis in Cerebral Parietal Cortex after Cerebral Ischemia Reperfusion in the Rats[J].Journal of Nanchang University(Natural Science),2009,33(1):1. |
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| Authors: | GU Qing WAN Yong WU Di YAO Li-xi YANG Li |
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| Institution: | College of Life Sciences;Nanchang University;Nanchang 330031;China |
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| Abstract: | To analyze the cell apoptosis changes in parietal cortex after cerebral ischemia-reperfusion in the rats,Twenty-four SD rats were randomly divided into four groups:control group,cerebral ischemia reperfusion of 6 hour,12 hour and 24 hour groups.Cerebral ischemia reperfusion model was performed by ligation of the left common carotid artery and clamping of the right common carotid artery for 15min.The changes of cell apoptosis were observed by Hoechst33258 fluorescein stain and Bax immunohistochemical method,... |
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| Keywords: | Bax Hoechst33258 |
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