紫外可见光谱法研究脂质体结构形态与染料的相互作用

本文利用紫外可见光谱研究了脂质体的三种特定结构：胶束，层状相，反六方相与染料的相互作用。通过光谱的变化可检验脂质体的形态结构，筛选出4种可用于检测不同脂质体结构的染料。初步探索出一种利用染料检测脂质体结构相态的方法并探讨了染料与脂质体不同相态的作用机理。该方法有望作为一种简便快捷的检测手段，在分离天然磷脂及生产过程中控制脂质体形状结构中得到应用。

Study of Interaction between the Phase States of Liposome and Dyes by UV-visible Absorption Spectra

Liposome is one of the major areas of interest in recent years because of its many potential applications. In this paper, three phase states of liposome (micelle, lamellar and reversed hexagonal) were prepared by thin film hydration method and their structures were observed with electron microscope. We studied the interaction between these phase states and various dyes by UV visible spectra. As a result, the phase states of liposome can be detected by the color change or the spectroscopic change. Through careful selection, 4 kinds of useful dyes were found to detect different liposome structures, that is 2,6 dichloroindophenol sodium, congred, pinacyanol chloride and calcein. The possible mechanism of the interaction between the phases of liposome and dyes was discussed. The liposome undertakes hydrolysis and forms mixed micelle. The hydrolysis rate of lamellar is faster than that of reversed hexagonal, which makes pH of the solution to decrease and causes a color change of the dyes in a short time. This method can be applied to the separation of natural phosphatides and monitor in the production of liposome.