光聚合法快速制备甲基丙烯酸酯类毛细管整体柱

采用甲基丙烯酸正丁酯(BMA)为功能单体, 乙二醇二甲基丙烯酸酯(EDMA)为交联剂, 正丙醇、1,4-丁二醇和水为致孔剂, Irgacure 1800为引发剂, 在毛细管内采用光引发原位聚合150 s快速制备了有机聚合物整体柱. 分别采用电色谱(CEC)、加压电色谱(p-CEC)和低压色谱(LPLC)模式对所制备的整体柱进行了性能评价, 基线分离了硫脲、甲苯、萘和联苯, 在加压电色谱(p-CEC)模式下硫脲的最低理论塔板高度达到了8.0 μm. 扫描电镜结果表明, 整体材料在毛细管柱中形成并与毛细管内壁结合紧密.

Rapid Preparation of Methacrylate Capillary Monolithic Columns by Photopolymerization

An organic-based monolithic column was quickly prepared inside the fused-silica capillary, and in situ photopolymerization was carried out in 150 s, using butyl-methacrylate(BMA) as a functional monomer, ethylene glycol dimethacrylate(EDMA) as a cross-linking agent, 1-propanol, 1,4-butanediol and water as porogenic solvents and Irgacure 1800 as an initiator. The electrophoretic and chromatographic behavior was comparatively evaluated using pressure-assisted CEC(p-CEC), CEC and low pressure-assisted liquid chromatography modes(LPLC). Baseline separation of the model analytes was respectively achieved including thiourea, toluene, naphthalene and biphenyl with the lowest theoretical height up to 8.0 μm for thiourea in the mode of p-CEC. A scanning electron micrograph of a cross-section of the capillary column shows that the monolithic columms were formed in the center of the capillary and adhered to the column inner wall.