| 保护氨基酸与羟基树脂的连接反应 |
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| 引用本文： | 王卫国,陈伟华,闻峰,李佩华,杨明,沈树宝.保护氨基酸与羟基树脂的连接反应[J].南京工业大学学报(自然科学版),2007,29(1):12-15. |
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| 作者姓名： | 王卫国 陈伟华 闻峰 李佩华 杨明 沈树宝 |
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| 作者单位： | 1. 南京工业大学,制药与生命科学学院,江苏,南京,210009
2. 嘉兴职业技术学院,生物环境系,浙江,嘉兴,314001 |
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| 基金项目： | 国家高技术研究发展计划(863计划)
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南京工业大学校科研和教改项目 |
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| 摘 要： | 在多肽固相合成中,分别采用酰氯法和活化酯法,研究了20种Fmoc氨基酸与Wang树脂的连接反应.除精氨酸外,其他氨基酸与树脂的连接效率均能达到80%以上.酰氯法反应速率快、操作简便,优于活化酯法.在酰氯法中,对于精氨酸,选择弱极法溶剂如DCM,延长反应时间至20h,连接效率接近80%.
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| 关 键 词： | Fmoc-氨基酸 Wang树脂 酰氯 活化酯 保护氨基酸 羟基树脂 连接效率 反应时间 resin linked amino acid 延长 溶剂 选择 操作 反应速率 精氨酸 Wang Fmoc 研究 活化酯法 酰氯法 多肽固相合成 |
| 文章编号： | 1671-7627(2007)01-0012-04 |
| 修稿时间： | 2006-06-02 |
Reaction of the protected amino acid linked to the hydroxy resin |
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| WANG Wei-guo,CHEN Wei-hua,WEN Feng,LI Pei-hua,YANG Ming,SHEN Shu-bao.Reaction of the protected amino acid linked to the hydroxy resin[J].Journal of Nanjing University of Technology,2007,29(1):12-15. |
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| Authors: | WANG Wei-guo CHEN Wei-hua WEN Feng LI Pei-hua YANG Ming SHEN Shu-bao |
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| Institution: | 1. College of Life Science and Pharmaceutical Engineering, Nanjing University of Technology, Nanjing 210009, China; 2. Department of Biology and environment, Jiaxing College of Vocation and Technique, Jiaxing 314001, China |
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| Abstract: | The reaction of twenty kinds of Fmoc- AA -OH linked to Wang resin using methods of acyl chloride or active ester was studied in solid-phase peptide synthesis. Expect for arginine, the yield of other amino acid linked to Wang resin was over 80%. The method of acyl chloride was better than the method of active ester for its high efficiency and convenient operation. For arginine, the yield was improved to nearly 80% by using weakly polar solvent such as DCM and simultaneously increasing the reaction time to 20 h in the method of acyl chloride. |
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| Keywords: | Fmoc-amino acid Wang resin acyl chloride active ester |
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