18β-Glycyrrhetinic acid interaction with bovine serum albumin |
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Institution: | 1. Research Center of Modernization of Chinese Medicines, College of Chemistry and Chemical Engineering, Central South University, Changsha 410083, China;2. Departments of Chemistry and Biology, YuLin Teachers College, YuLin GuangXi 537000, China |
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Abstract: | The interaction of 18β-glycyrrhetinic acid (GA): The metabolite of glycyrrhizic acid which is the main active component of a commonly used traditional Chinese medicine (TCM) Glycyrrhiza Uralensis Fisch with bovine serum albumin (BSA) has been investigated. Fluorescence emission spectra of serum albumin in the presence of GA, recorded at the excitation wavelength 280 nm, clearly show that GA act as quencher and have different quenching mechanism at a pH below or above the isoelectric point (pI). The binding sites number n and apparent binding constant K were measured. The thermodynamic parameters ΔH°, ΔG°, ΔS° at different temperatures were calculated. The effects of some common metal ions on binding are considered. Synchronous fluorescence and UV–vis spectra were used to study protein conformation. Energy transfer between GA and HSA was calculated by Förster's theory and the binding site was suggested to be site II. The binding of monoammonium glycyrrhizinate (GL) to BSA is also compared. |
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