High-speed wave-mixing laser Doppler imaging in vivo |
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Authors: | Atlan M Gross M Vitalis T Rancillac A Rossier J Boccara A C |
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Institution: | Laboratoire Kastler-Brossel de l'Ecole Normale Supérieure, CNRS UMR 8552, Université Pierre et Marie Curie - Paris 6, 24 rue Lhomond, 75231 Paris CEDEX 05, France. atlan@lkb.ens.fr |
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Abstract: | An interferometric method for parallel optical spectroscopy in the kilohertz range is reported, as well as its experimental validation in the context of high-speed laser Doppler imaging in vivo. The interferometric approach enables imaging in the low light conditions of a 2 kHz frame rate recording with a complementary metal-oxide semiconductor camera. Observation of mice craniums with near-infrared (lambda=785 nm) laser light in reflection configuration is reported. Doppler spectral images allegedly sensitive to blood flow are sequentially measured at several optical frequency detunings, to shift the spectral range of analysis in the radio-frequency spectrum. |
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