Control of charcoal rot fungus Macrophomina phaseolina by extracts of Datura metel |
| |
Authors: | Javaid Arshad Saddique Amna |
| |
Institution: | Institute of Agricultural Sciences, University of the Punjab, Lahore, Pakistan. arshadjpk@yahoo.com |
| |
Abstract: | Methanolic leaf and fruit extracts of Datura metel were found highly effective in suppressing against Macrophomina phaseolina, the cause of charcoal rot disease. These extracts were further subjected to successive fractionation with n-hexane, chloroform, ethyl acetate and n-butanol. All the concentrations (3.125-200?mg?mL?1) of chloroform, ethyl acetate and n-butanol fractions of leaf extract, and n-hexane fraction of fruit extract completely inhibited the target fungal growth. Two compounds A and B from the n-hexane fraction of fruit extract and compound C from n-butanol fraction of leaf extract were obtained by TLC. Compound B exhibited the best antifungal activity with an MIC value of 7.81?μg?mL?1 that was at par with that of commercial fungicide mancozeb (80% w/w). This study concludes that M. phaseolina can be effectively controlled by natural antifungal compounds in n-hexane fraction of methanolic fruit extract of D. metel. |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |
|