衰减全反射红外光谱用于人乳腺癌组织的研究

通过对32例人乳腺良、恶性肿瘤的衰减全反射红外光谱的对比研究发现，它们之间存在明显而规律的光谱差异，反映了乳腺良、恶性病变组织中，蛋白质、核酸和脂类等生物大分子的构型与构象以及相对含量上的差别.研究表明:傅里叶变换红外光谱可以从分子水平上揭示良、恶性肿瘤的分子结构信息，极有潜力成为一种临床原位诊断肿瘤的新方法.

Studies on Breast Cancer Tissues With ATR-FTIR Spectroscopy

Attenuated total reflection (ATR) FTIR spectra of benign and malignant breast tumor tissues from 32 female patients have been determined and investigated. The spectral differences and changes (shape, peak position and relative intensities) have been observed between these two types of samples. In comparison with the benign tumor tissues, the mainly characteristic changes of malignant breast tissues involve: 1) The secondary structure of proteins in malignant tumor tissues is composed mainly of α-helix and random coil, in which α-helix structure is dominant. 2) The absorption band near 1160 cm~-1 due to the C-O(H) stretching modes of serine, threonine, tyrosine groups evidently shifted to a higher wavenumber, suggesting that the intermolecular hydrogen bonds were nearly broken, and the collagen absorption peaks at 1205 cm~-1 , 1278 cm~-1 and 1338 cm~-1 became lower or disappeared, showing its relative contents decreased. 3) The bands from the symmetric and asymmetric stretching modes of the PO-_2 of the phosph