An anti E. coli O157:H7 antibody-immobilized microcantilever for the detection of Escherichia coli (E. coli). |
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Authors: | Jing Zhang Hai-Feng Ji |
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Affiliation: | Department of Chemistry, Institute for Micromanufacturing, Louisiana Tech University, Ruston, Louisiana 71272, USA. |
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Abstract: | A silicon microcantilever sensor was developed for the detection of Escherichia coli O157:H7. The microcantilever was modified by anti-E. coli O157:H7 antibodies on the silicon surface of the cantilever. When the aquaria E. coli O157:H7 positive sample is injected into the fluid cell where the microcantilever is held, the microcantilever bends upon the recognition of the E. coli O157:H7 antigen by the antibodies on the surface of the microcantilever. A negative control sample that does not contain E. coli O157:H7 antigen did not cause any bending of the microcantilever. The detection limit of the sensor was 1 x 10(6) cfu/mL when the assay time was < 2 h. |
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