| 激光单分子探测的实验研究 |
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| 引用本文： | 张亮,刘岗,马万云,文克玲,赵峰,王树虎,陈瓞延. 激光单分子探测的实验研究[J]. 光谱学与光谱分析, 1998, 18(6): 641-644 |
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| 作者姓名： | 张亮 刘岗 马万云 文克玲 赵峰 王树虎 陈瓞延 |
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| 作者单位： | 清华大学现代应用物理系,单原子分子测控中心,100084,北京 |
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| 摘 要： | 单分子探测技术是一项超高灵敏度的探测技术，在生物、医学和环境等领域有着广泛的应用。在这里，本文简要介绍了基于激光诱发荧光方法的单分子探测谱仪的原理和装置，以及用ＣＣ５染料分子在该探测谱仪上进行的若干实验研究结果，包括样品流速的影响、能量特性、浓度线性、检测限等方面，并讨论了光漂白现象对实验结果的影响。
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| 关 键 词： | 单分子探测 激光诱发荧光 光漂白 C445 染料分子 |
EXPERIMENTAL RESEARCH ON LASER-INDUCED SINGLE-MOLECULE DETECTION |
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| ZHANG Liang,LIU Gang,MA Wanyun,WEN Keling,ZHAO Feng,WANG Shuhu,CHEN Dieyan. EXPERIMENTAL RESEARCH ON LASER-INDUCED SINGLE-MOLECULE DETECTION[J]. Spectroscopy and Spectral Analysis, 1998, 18(6): 641-644 |
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| Authors: | ZHANG Liang LIU Gang MA Wanyun WEN Keling ZHAO Feng WANG Shuhu CHEN Dieyan |
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| Affiliation: | Center of Atomic and Molecular Sciences, Department of Physics, Tsinghua University, 100084 Beijing. |
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| Abstract: | Single molecule detection is a kind of ultra high sensitive detection technique.It is widely used in biology,medicine,environment and so on.Here we report briefly the principle and device of our laser induced signle molecule detection spectrum instrument.We also report the experimental results on the instrument by using C445 dye,including the affection of sample velocity,energy characteristic,consistence linearity and detection limit.We discuss the effect of photon bleach on experimental results.These results are the base of our future work. |
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| Keywords: | Single molecule detection Laser induced fluorescence Photon bleach C445 |
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