Simultaneous immobilization of glucose oxidase and peroxidase to urea derivative of regenerated acetylcellulose granules |
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Authors: | Lyubov K Yotova Ivan P Ivanov |
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Institution: | (1) Department of Biotechnology, Higher Institute of Chemical Technology, 1756 Sofia, Bulgaria;(2) Department of Chemistry and Biochemistry, Higher Institute of Medicine, Zdrave Str. 2, 1431 Sofia, Bulgaria |
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Abstract: | A method for simultaneous covalent immobilization of glucose oxidase and peroxidase with previously oxidized carbohydrate
residues to urea derivative of regenerated acetylcellulose granules is described. The effect of immobilization on the catalytic
properties of the separately immobilized enzymes are studied. The immobilized enzymes manifested no change in their pH and
temperature optima and slight increase ofK
m
x compared to data for the soluble enzymes. A column packed with simultaneously immobilized enzymes is used for manual glucose
determination in blood sera. The results are in high correlation with those obtained by the Beckman Glucose Analyzer method
(r = 0.976). The method is economic (the enzyme-carrier conjugate may be used more than 300 times), easy to perform, and less
time consuming than the manual methods utilizing soluble enzymes. The established manual method can be proposed for emergency
clinical analysis and smaller clinical laboratories. |
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Keywords: | Simultaneous immobilization glucose oxidase peroxidase determination of glucose urea derivative of cellulose |
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