The requirement of natural killer T-cells in tolerogenic APCs-mediated suppression of collagen-induced arthritis |
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Authors: | Sundo Jung Yoon-Kyung Park Jung Hoon Shin Hyunji Lee Soo-Young Kim Gap Ryol Lee and Se-Ho Park |
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Institution: | 1School of Life Sciences and Biotechnology, Korea University, Seoul 136-701, Korea.;2Department of Anatomy, Division of Brain Korea 21, Biomedical Science, Korea University College of Medicine, Seoul 136-705, Korea.;3Department of Life Science, Sogang University, Seoul 121-742, Korea. |
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Abstract: | TGF-β-induced tolerogenic-antigen presenting cells (Tol-APCs) could induce suppression of autoimmune diseases such as collagen-induced arthritis (CIA) and allergic asthma. In contrast, many studies have shown that NKT cells are involved in the pathogenesis of Th1-mediated autoimmune joint inflammation and Th2-mediated allergic pulmonary inflammation. In this study, we investigated the effect of CD1d-restricted NKT cells in the Tol-APCs-mediated suppression of autoimmune disease using a murine CIA model. When CIA-induced mice were treated with Tol-APCs obtained from CD1d+/- or CD1d-/- mice, unlike CD1d+/- APCs, CD1d-/- Tol-APCs failed to suppress CIA. More specifically, CD1d-/- Tol-APCs failed to suppress the production of inflammatory cytokines and the induction of Th2 responses by antigen-specific CD4 T cells both in vitro and in vivo. Our results demonstrate that the presence of CD1d-restricted NKT cells is critical for the induction of Tol-APCs-mediated suppression of CIA. |
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Keywords: | antigen-presenting cells antigens arthritis CD1d experimental immune tolerance natural killer T-cells |
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