A microfluidic electrochemical device for high sensitivity biosensing: Detection of nanomolar hydrogen peroxide |
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| Authors: | Bhaskara V. Chikkaveeraiah Hongyun Liu Vigneshwaran Mani Fotios Papadimitrakopoulos James F. Rusling |
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| Affiliation: | 1. Department of Chemistry, Institute of Materials Science, University of Connecticut, 55 N. Eagleville Rd., U-60, Storrs, CT 06269-3060, USA;2. Cell Biology, University of Connecticut Health Center, Farmington, CT 06032, USA;3. Department of Chemistry, Beijing Normal University, Beijing 100875, China;4. School of Chemistry, National University of Ireland at Galway, Ireland |
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| Abstract: | We report herein a simple device for rapid biosensing consisting of a single microfluidic channel made from poly(dimethylsiloxane) (PDMS) coupled to an injector, and incorporating a biocatalytic sensing electrode, reference and counter electrodes. The sensing electrode was a gold wire coated with 5 nm glutathione-decorated gold nanoparticles (AuNPs). Sensitive detection of H2O2 based on direct bioelectrocatalysis by horseradish peroxidase (HRP) was used for evaluation. HRP was covalently linked the glutathione–AuNPs. This electrode presented quasi-reversible cyclic voltammetry peaks at ?0.01 V vs. Ag/AgCl at pH 6.5 for the HRP heme FeIII/FeII couple. Direct electrochemical activity of HRP was used to detect H2O2 at high sensitivity with a detection limit of 5 nM in an unmediated system. |
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