Bell's expression and the generalized Garg form for forced dissociation of a biomolecular complex |
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Authors: | Lin Han-Jou Chen Hsuan-Yi Sheng Yu-Jane Tsao Heng-Kwong |
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Affiliation: | Department of Chemical Engineering, National Taiwan University, Taipei, Taiwan 106, Republic of China. |
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Abstract: | The dissociation of a biomolecular complex under the action of constant force, constant loading rate, and periodic force is studied theoretically. We show that the celebrated Bell expression provides a good approximation for the bond dissociation rate when F/F(c)<1, where F(c) is the maxima slope of the binding potential along the reaction coordinate. When 1-F/F(c) <1 the dissociation rate is better described by a generalized Garg form in which the potential derivative is expanded near F(c). We also show that a constant-force experiment is suitable for extracting the activation energy of the bond, a constant loading experiment is suitable to extract F(c), while time-periodic force can be applied to extract both bond dissociation rates at zero force and F(c). |
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