Ion-sensitive field effect transistor as a monovalent cation detector for ion chromatography and its application to the measurement of Na+ and K+ concentrations in serum |
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Authors: | K Watanabe K Tohda H Sugimoto F Eitoku H Inoue K Suzuki S Nakamura |
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Affiliation: | Department of Applied Chemistry, Faculty of Science and Technology, Keio University, Yokohama, Japan. |
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Abstract: | An ion-sensitive field effect transistor, which is a small potentiometric ion-sensing probe, was applied as a detector in the ion chromatography of alkali-metal cations. The cation-sensing transistor was prepared by coating the gate part of the probe to form a poly(vinyl chloride) matrix membrane containing tetranactin, which enables detection of alkali-metal ions such as Na+, K+, Rb+, and Cs+. To be able to analyse amounts less than 1 microliter and make full use of the small ion-sensing probe, we constructed a miniature cation-exchange separation column (support 10 microns polystyrene) with a PTFE tube (20 mm x 1.5 mm I.D. x 2.1 mm O.D). As an application of the system, Na+ and K+ concentrations in serum were determined. The analytical results for these two cations were good agreement with those obtained by flame photometry. |
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