An integration method of enzyme analysis

The application of an integration method of kinetic analysis to first-order and second-order reactions is discussed with particular emphasis on enzyme analyses. Transducer signals or concentrations of products or substrates are integrated for a Fixed time and the net integral of the increased or decreased signal or concentration is related to the initial substrate or enzyme concentration. Equations are developed describing the dependence of the integrals on enzyme and substrate concentrations for first- and second-order reactions, and examples are presented illustrating different cases. The application of this method to complicated enzymatic systems is discussed.