Portable microfluidic system for determination of urinary creatinine |
| |
Authors: | Temsiri Songjaroen Aussawapong Sappat Wanida Laiwattanapaisal |
| |
Affiliation: | a Graduate Program in Clinical Biochemistry and Molecular Medicine, Faculty of Allied Health Sciences, Chulalongkorn University, Bangkok, 10330, Thailand b Nanoelectronics and MEMS Laboratory, National Electronics and Computer Technology Center, Pathum Thani, 12120, Thailand c Center for Excellence in Omics-Nano Medical Technology Project Development, Department of Clinical Chemistry, Faculty of Allied Health Sciences, Chulalongkorn University, Bangkok, 10330, Thailand |
| |
Abstract: | A simple, low cost and portable microfluidic system based on a two-point alkaline picrate kinetic reaction has been developed for the determination of urinary creatinine. The creatinine reacts with picric acid under alkaline conditions, forming an orange-red colour, which is monitored on PDMS microchip using a portable miniature fibre optic spectrometer at 510 nm. A linear range was displayed from 0 to 40 mg L−1 creatinine (r2 = 0.997) with a detection limit of 3.3 mg L−1 (S/N = 3). On-chip absorbance signals are reproducible, with relative standard deviations (RSDs) of 7.1%, when evaluated with 20 mg L−1 creatinine (n = 10). The standard curves in which the intra-run CVs (4.7-6.8%) and inter-run CVs (7.9%) obtained were performed on three different days and exhibited good reproducibility. The method was highly correlated with the conventional spectrophotometric method when real urine samples were evaluated (r2 = 0.948; n = 15). |
| |
Keywords: | Creatinine determination Microfluidic system Poly(dimethylsiloxane) Jaffé reaction On-chip absorbance detection |
本文献已被 ScienceDirect 等数据库收录! |
|