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Analysis of EU priority polycyclic aromatic hydrocarbons in food supplements using high performance liquid chromatography coupled to an ultraviolet, diode array or fluorescence detector |
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| Authors: | Sophie Danyi François Brose Yves-Jacques Schneider Luc Pussemier Sarah De Saeger Marie-Louise Scippo |
| Institution: | a Department of Food Sciences, Laboratory of Food Analysis, Faculty Of Veterinary Medicine, CART (Centre of Analysis of Residue in Traces), University of Liège, B43b, Boulevard de Colonster 20, Sart-Tilman, B-4000 Liège, Belgium b Laboratoire de Biochimie cellulaire, nutritionnelle et toxicologique, Institut des Sciences de la Vie & Université catholique de Louvain, Croix du Sud, B 1348 Louvain-la-Neuve, Belgium c CERVA-CODA, Veterinary and Agrochemical Research Centre, Leuvensesteenweg 17, B-3080 Tervuren, Belgium d Laboratory for Ecophysiology, Biochemistry and Toxicology, Department of Biology, University of Antwerp, Groenenborgerlaan, 171 B-2020, Antwerp, Belgium e Laboratory of Food Analysis, Faculty of Pharmaceutical Sciences, University Ghent Harelbekestraat 72, B 9000 Gent, Belgium |
| Abstract: | High performance liquid chromatography coupled to an ultraviolet, diode array or fluorescence detector (HPLC/UV-FLD) has been used to set up a method to detect the 15(+1) EU priority polycyclic aromatic hydrocarbons (PAHs) in food supplements covering the categories of dried plants and plant extracts excluding oily products. A mini validation was performed and the following parameters have been determined: limit of detection, limit of quantification, precision, recovery and linearity. They were in close agreement with quality criteria described in the Commission Regulation (EC) No 333/2007 concerning the PAH benzoa]pyrene in foodstuffs, except the not fluorescent cyclopentac,d]pyrene for which the UV detection leads to a higher limit of detection. Analysis of twenty commercial food supplements covering mainly the class of dried plants was performed to evaluate their PAHs contamination levels and to test the applicability of the method to various plant matrices. Fifty percent of analyzed samples showed concentration exceeding 2 μg kg−1 for one or more PAHs. |
| Keywords: | Polycyclic aromatic hydrocarbons High performance liquid chromatography Fluorescence detection Food supplement Analytical procedure validation |
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