Langmuir-Blodgett film based on MEH-PPV for cholesterol biosensor |
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| Authors: | Zimple Matharu Sunil K Arya Vinay Gupta |
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| Institution: | a Biomolecular Electronics and Conducting Polymer Research Group, National Physical Laboratory, Dr. K.S. Krishnan Marg, New Delhi 110012, India
b Department of Physics and Astrophysics, University of Delhi, Delhi 110007, India |
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| Abstract: | Cholesterol oxidase (ChOx) has been immobilized onto conducting poly2-methoxy,5-(2′-ethyl-hexyloxy)-1,4-phenylene vinylene] (MEH-PPV)/stearic acid (SA) Langmuir-Blodgett film transferred onto octadecanethiol (ODT) modified gold plate. The ChOx/MEH-PPV/SA LB film bioelectrode exhibits has been characterized by FT-IR, contact angle, and atomic force microscopy. The response of the ChOx/MEH-PPV/SA LB film bioelectrode carried out using differential pulse voltammetry (DPV) studies reveal linearity from 1.29 to 12.91 mM of cholesterol concentration and response time as 30 s. This ChOx/MEH-PPV/SA bioelectrode exhibits values of correlation coefficient as 0.9939, standard deviation as 0.0029 μA and limit of detection as 1.66 mM. UV-visible spectrophotometer studies reveal that 5.2 × 10−3 U of ChOx are actively working per cm2 area of ChOx/MEH-PPV/SA LB film bioelectrode and this bioelectrode is thermally stable upto 55 °C with reusability of about 60 times. |
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| Keywords: | Langmuir-Blodgett film poly[2-methoxy 5-(2&prime -ethyl-hexyloxy)-1 4-phenylene vinylene] Cholesterol oxidase Differential pulse voltammetry Ultraviolet-visible |
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