Optimizing the performance of tin dioxide microspheres for phosphopeptide enrichment |
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Authors: | Alexander Leitner,Martin Sturm,Mikael Jä rn,Karl Mechtler |
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Affiliation: | a Department of Analytical Chemistry and Food Chemistry, University of Vienna, Waehringer Strasse 38, 1090 Vienna, Austria b Center for Functional Materials, Department of Physical Chemistry, Åbo Akademi University, Porthansgatan 3-5, 20500 Turku, Finland c Research Institute of Molecular Pathology, Dr. Bohrgasse 7, 1030 Vienna, Austria |
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Abstract: | Phosphopeptide enrichment based on metal oxide affinity chromatography is one of the most powerful tools for studying protein phosphorylation on a large scale. To complement existing metal oxide sorbents, we have recently introduced tin dioxide as a promising alternative. The preparation of SnO2 microspheres by the nanocasting technique, using silica of different morphology as a template, offers a strategy to prepare materials that vary in their particle size and their porosity. Here, we demonstrate how such stannia materials can be successfully generated and their properties fine-tuned in order to obtain an optimized phosphopeptide enrichment material. We combined data from liquid chromatography-mass spectrometry experiments and physicochemical characterization, including nitrogen physisorption and energy-dispersive X-ray spectroscopy (EDX), to explain the influence of the various experimental parameters. |
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Keywords: | Phosphorylation Phosphopeptide enrichment Metal oxide affinity chromatography Tin dioxide Mass spectrometry Nanocasting |
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