Sensitive and rapid chemiluminescence enzyme immunoassay for microcystin-LR in water samples |
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Authors: | F. Long H.C. Shi M. He J.W. Sheng J.F. Wang |
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Affiliation: | Environmental Simulate and Pollution Control State Key Joint Laboratory, Department of Environment Science and Engineering, Tsinghua University, Beijing 100084, China |
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Abstract: | A highly sensitive, specific, simple, and rapid chemiluminescence enzyme immunoassay (CLEIA) was developed for the determination of microcystin-LR (MC-LR). Several physicochemical parameters such as the chemiluminescent assay mediums, the dilution ratio of MC-LR-OVA conjugate, monoclonal antibody concentration, and peroxidase labeled antibody concentration were studied and optimized. Under optimum conditions, calibration curve obtained for MC-LR had detection limits of 0.032 ± 0.003 μg L−1, the 50% inhibition concentration (IC50) was 0.20 ± 0.02 μg L−1 and the quantitative detection range was 0.062-0.65 μg L−1. The proposed methods was successfully applied to the monitoring of MC-LR in spiked water samples without significant effect of the matrix, and the recovery of MC-LR added to water samples at different concentrations ranged from 80% to 115% with the coefficients of variation (CVs) less than 9%. The LOD attained from the calibration curves and the results obtained for the real samples demonstrate the potential use of CLEIA as a screening tool for the analysis of MC-LR in environmental samples. |
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Keywords: | Chemiluminescence Enzyme immunoassay Microcystin-LR Environmental analysis |
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