Determination of cyanide, in urine and gastric content, by electrospray ionization tandem mass spectrometry after direct flow injection of dicyanogold |
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Authors: | Kayoko Minakata Hideki Nozawa Masako Suzuki |
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Institution: | a Department of Legal Medicine, Hamamatsu University School of Medicine, 1-20-1 Handayama, Hamamatsu 431-3192, Japan b Research Equipment Center, Hamamatsu University School of Medicine, 1-20-1 Handayama, Hamamatsu 431-3192, Japan |
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Abstract: | A rapid and sensitive electrospray ionization tandem mass spectrometric (ESI-MS-MS) procedure was developed for the determination of cyanide (CN−). CN− in biological fluids was reacted with NaAuCl4 to produce dicyanogold, Au(CN)2−, which was extracted with methyl isobutyl ketone (MIBK). One microliter of the extract was injected directly into an ESI-MS-MS instrument. Quantification of CN− was performed by selected reaction monitoring of the product ion CN− at m/z 26 that derived from precursor ion Au(CN)2− at m/z 249. CN− could be measured in the quantification range of 10−7 to 5 × 10−5 M with the limit of detection at 4 × 10−8 M using 10 μL of urine within 10 min. A victim's urine and gastric content were diluted with water to 4-fold and 500-fold and measured, respectively. |
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Keywords: | Cyanide Gold Electrospray ionization Tandem mass spectrometry Urine |
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