Synthesis and characterization of two Cu(II) complexes with a new pyrazole-based Schiff base ligand: crystallography,DNA interaction and antimicrobial activity of Ni(II) and Cu(II) complexes |
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| Authors: | Susmita Mandal Ratan Sadhukhan Utpal Ghosh Soumik Mandal Manan Saha Ray J Butcher |
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| Institution: | 1. Department of Chemistry, University of Kalyani, Kalyani, India;2. Department of Biochemistry &3. Biophysics, University of Kalyani, Kalyani, India;4. Department of Chemical Sciences, Indian Institute of Science Education and Research, Kolkata, India;5. Department of Chemistry, Howard University, Washington, DC, USA |
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| Abstract: | Reaction of Cu(II) nitrate with a new pyrazole-based Schiff base ligand, 5-methyl-3-formylpyrazole-N-(2′-methylphenoxy)methyleneimine (MPzOA), afforded two types of Cu(II) complexes at different reaction temperatures, Cu(MPzOA)(NO3)]2 (1) and Cu(3,7,11,15-tetramethylporphyrin)(H2O)](NO3)2 (2), reported together with a Ni(II) complex, Ni(MPzOA)2(H2O)2]Br2 (3). The compounds are characterized by single crystal X-ray structure analyses along with several physico-chemical and spectral parameters. Complex 1 is authenticated as a bis(μ-pyrazolato)dicopper(II), while 2 is a porphyrinogen and 3 is a distorted octahedral complex. Structural analyses of the complexes reveal that 1 crystallized in monoclinic P21/n space group while 2 and 3 crystallized in monoclinic C2/c space group. DNA-binding studies of the complexes have shown that the complexes interact with CT-DNA. DNA-cleavage studies with plasmid DNA have shown that 1 and 2 induce extensive DNA cleavage in the presence of H2O2 as an additive, whereas there is no change in degradation of super-coiled DNA by 3 in the presence of additive. The antimicrobial studies of the complexes against Escherichia coli DH5α bacteria strain indicated that all the complexes were capable of killing E. coli with different LD50 values. |
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| Keywords: | Schiff base ligand pyrazole Ni(II) Cu(II) DNA interaction antimicrobial activity |
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