Automated headspace solid‐phase microextraction and on‐fiber derivatization for the determination of clenbuterol in meat products by gas chromatography coupled to mass spectrometry |
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Authors: | Yong Jiang Yongnian Ni |
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Institution: | 1. School of Life Science, Jiangxi Science and Technology Normal University, Nanchang, P. R. China;2. State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang, P. R. China |
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Abstract: | A method was developed for the determination of clenbuterol in meat using stable‐isotope‐dilution gas chromatography with mass spectrometry coupled with solid‐phase microextraction and on‐fiber derivatization. The samples were first homogenized with hydrochloric acid followed by protein deposition. After headspace solid‐phase microextraction and on‐fiber derivatization, the content of clenbuterol was measured with the aid of stable‐isotope dilution. The condition of solid‐phase microextraction was optimized by central composite design. The relative standard deviations, limit of detection, and recoveries for clenbuterol were 4.2–9.2%, 0.48 μg/kg, and 96–104%, respectively. The proposed method was satisfactory for analysis of real samples as compared with the Chinese standard method. |
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Keywords: | Clenbuterol Derivatization Gas chromatography with mass spectrometry Meat products Solid‐phase microextraction |
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