叶绿体光诱导延迟荧光产生机理的光谱学研究

研究延迟荧光产生机理对延迟荧光的应用具有重要指导意义。从电荷复合理论出发，对叶绿体光诱导延迟荧光的产生机制进行了简化的理论模拟。得出： 延迟荧光的衰减动力学符合双指数形式;衰减中快相成分对应电子从Q-_A上电子回流与P680+的复合产生;慢相成分对电子从Q=_B回流与P680+的复合产生。实验结果支持上述理论推理，并证明延迟荧光的更长延时成分来源于PSⅠ中的电子回流到PSⅡ与P680+复合产生激发态的P680*退激发产生。

Spectroscopy Research on the Origin Mechanism for Light Induced Delayed Fluorescence of Chloroplast

Research of the mechanism for delayed fluorescence origin play an important role in the application of DF. Based onthe charge recombination theory, the mechanism for delayed fluorescence origin is theoretically analyzed in this paper. The decay kinetics of DF, depending on the backward transport electron and oxidized state P680+, can be well fitted by poly-exponent. The relative fast component of DF originates from the Z+ P680 Q(A)- states, generated during the previous period of the sample illumination. On the other hand, the slow component of DF is ascribed to the state Q(B)= --the next electron carrier of deoxidized side in PS II. Meanwhile, the experimental results confirmed the theoretical conclusion. Furthermore, the authors testified that the third component with lifetime much greater than the time of the registration period (which is presented as a constant), is corresponds to the recombination of P680+ and the backward transport electron from PSI.