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Fluorimetric determination of nucleic acid using the enhancement of terbium-gadolinium-nucleic acid-cetylpyridine bromide system
Authors:Li Lei  Yang Jinghe  Wu Xia  Sun Changxia  Zhou Guangjun
Institution:a Department of Chemistry, Laboratory of Colloid and Interface Chemistry for Educational Ministry, Shandong University, Jinan 250100, People's Republic of China
b Shandong Supervision and Inspection Institute for Product Quality, Jinan 250100, People's Republic of China
Abstract:It is found that the fluorescence intensity of Tb-cetylpyridine bromide (CPB)-nucleic acid system can be enhanced by La3+, Gd3+, Lu3+, Sc3+ and Y3+, of which Gd3+ has the greatest enhancement. The experiments indicate that under the optimum condition, the fluorescence intensity of the system is in proportion to the concentration of nucleic acids in the range from 9×10−8 to 1×10−5 g ml−1 for yeast RNA, from 1×10−7 to 1×10−5 g ml−1 for fish sperm DNA. The detection limits are 3.2 and 4.1 ng ml−1, respectively. This method has been used satisfactorily for the determination of both synthetic and actual samples. In comparison with most fluorescence method for the determination of nucleic acids, this method has higher sensitivity and stability.
Keywords:Nucleic acid  Fluorescence intensity  Cetylpyridine bromide
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