Biosensors for phenol derivatives using biochemical signal amplification |
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Authors: | Stanca Sarmiza Elena Popescu Ionel Catalin Oniciu Liviu |
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Affiliation: | a Department of Physics, Chemistry and Informatics, Faculty of Environmental Protection, University of Oradea, 3700 Oradea, Romania b Laboratory for Photonics and Interfaces, Instiute of Molecular and Biological Chemistry, Swiss Federal Institute of Technology, EPFL, ICMB LPI F1-496, Lausanne CH-1015, Switzerland c Department of Physical Chemistry, University “Babes-Bolyai”, 3400 Cluj-Napoca, Romania |
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Abstract: | Two different approaches, both exploiting two enzymes cooperative functioning, to enhance the sensitivity of tyrosinase (PPO) based biosensor for amperometric detection of phenols have been compared. For this purpose, one monoenzyme electrode (PPO) and two bienzyme electrodes (PPO and d-glucose dehydrogenase, GDH; PPO and horseradish peroxidase, HRP) were constructed using agar-agar gel as enzyme immobilization matrix. The biosensors responses for l-tyrosine detection were recorded at −50 mV versus saturated calomel electrode (SCE). The highest sensitivity (74 mA M−1) was observed for the PPO-GDH couple, while that recorded for PPO-HRP couple system was only 32 times higher than that measured for monoenzyme electrode (0.01 mA M−1). The ability of the PPO-, PPO-GDH-, PPO-HRP-based biosensors to assay phenols was demonstrated by quantitative determination of phenol, 1,2-dihydroxybenzene, 1,3-dihydroxybenzene, 1,4-dihydroxybenzene, 2-amino-3 (4-hydroxyphenyl) propanoic acid, 2-hydroxytoluene, 3-hydroxytoluene, 4-hydroxytoluene, 4-clorophenol, 3-clorophenol, 2-clorophenol, 4-hydroxybenzoic acid. |
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Keywords: | Biosensor signal amplification Phenols determination Tyrosinase Glucose dehydrogenase Horseradish peroxidase |
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