Determination of cadmium in biological samples solubilized with tetramethylammonium hydroxide by electrothermal atomic absorption spectrometry, using ruthenium as permanent modifier

The feasibility of Ru as a permanent modifier for the determination of Cd in biological samples treated with tetramethylammonium hydroxide (TMAH) by ET AAS was investigated. The tube treatment with Ru was carried out only once and lasted for about 300 atomization cycles. The pyrolysis and atomization temperatures, 750 °C and 1300 °C, respectively, were chosen from the temperature curves. The sample dissolution procedure was very simple: a sample aliquot was mixed with a small volume of a 25% m/v TMAH solution, the volume was made up to 50 ml and the mixture was kept at 60 °C for 1 h. Six certified biological reference materials were analyzed and the obtained Cd concentrations are within the 95% confidence interval of the certified values, proving the accuracy of the proposed procedure for a variety of biological samples. The calibration curve, with correlation coefficient higher than 0.99, was established for a working range up to10 μg l⁻¹. The precision was good as demonstrated by relative standard deviations below 3%, except for one sample. The limit of detection (3σ) was 0.05 μg l⁻¹ and the characteristic mass was 1.30 pg, obtained in the presence of the Ru modifier.