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Comparison of pyrolysis mass spectrometry with high performance liquid chromatography for the analysis of Cremophor EL in drugs and serum |
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| Authors: | Ghassempour Alireza Nabid Mohammad Reza Talebi Mohammad Entezami Ali Akbar |
| Affiliation: | a Chemical Research Center, Faculty of Science, Shahid Beheshti University, Evin, Tehran, Iran b Department of Chemistry, Faculty of Science, Shahid Beheshti University, Evin, Tehran, Iran c Faculty of Chemistry, Tabriz University, Tabriz, Iran |
| Abstract: | Polyoxyethyleneglycerol triricinoleate 35 (Cremophor EL: CrEL) is a solubilization agent for hydrophobic drugs. Recently, CrEL has shown some side effects in patients. In the present work, we introduce pyrolysis-mass spectrometry (Py-MS) for the determination of CrEL in drugs and blood samples. Mass to charge (m/z) values of 89 and 138 of CrEL and 3-nitroaniline (as internal standard), respectively, were used for quantitative measurements by selected ion monitoring (SIM) method. At a probe pyrolysis temperature range of 350-450 °C the results are highly reproducible. Limit of detection (LOD), linearity and relative standard deviation (R.S.D., n=5) were determined to be 1 ng ml−1, 10 ng ml−1-100 mg ml−1 and 1.3%, respectively. The results of Py-MS are compared with those obtained by high performance liquid chromatography (HPLC) and show that time for analysis, sensitivity and linearity are far better. |
| Keywords: | Pyrolysis Mass spectrometry Cremophor EL HPLC |
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