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Analysis of promazines in bovine livers by high performance liquid chromatography with ultraviolet and fluorimetric detection
Authors:Quintana M C  Blanco M H  Lacal J  Hernández L
Institution:Department of Analytical Chemistry and Instrumental Analysis, Faculty of Sciences, Universidad Autónoma de Madrid, E-28049 Madrid, Spain
Abstract:Fluorimetric and UV methods of detection for liquid chromatographic determination of phenothiazines (chlorpromazine, acetopromazine and propionylpromazine) were developed. The effects of several experimental parameters on the separation and the sensitivity of the methods were evaluated. The detection limits ranged from 31 to 350 ng ml−1. Optimized methods were successfully applied to determination of the promazines in bovine liver samples.
Keywords:Phenothiazines  Ultraviolet  Fluorescence  Bovine livers
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