Development of a chemiluminescent optical fiber immunosensor to detect Streptococcus pneumoniae antipolysaccharide antibodies |
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| Authors: | Robert S Marks Alon Margalit Alexei Bychenko Efim Bassis Nurith Porat Ron Dagan |
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| Institution: | (1) Unit of Biotechnology, Soroka University and the Faculty of Health Sciences, Ben-Gurion University of the Negev, PO Box 653, 84105 Beer-Sheva, Israel;(2) Institute for Applied BioSciences, Soroka University and the Faculty of Health Sciences, Ben-Gurion University of the Negev, PO Box 653, 84105 Beer-Sheva, Israel;(3) Pediatric Infectious Disease Unit, Soroka University and the Faculty of Health Sciences, Ben-Gurion University of the Negev, PO Box 653, 84105 Beer-Sheva, Israel |
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| Abstract: | A chemiluminescent-based optical fiber immunosensor was developed for the detection of antipneumococcal antibodies. This was
accomplished by developing a different chemical procedure utilizing 3-aminopropyl trimethoxysilane and cyanuric chloride to
conjugate pneumococcal cell wall polysaccharides to the optical fiber tips, and by improving the sensitivity of the photodetection
system. The lowest titer of antipneumococcal antibodies detected by the optical fiber was at a 1:819,200 dilution. The lowest
corresponding value by standard enzyme-linked immunosorbent assay was at a 1:98,415 dilution. It was concluded that the optical
immunosensor system is an accurate and sensitive method to detect antipneumococcal antibodies and may be an adequate tool
to monitor antibodies in specimens such as saliva and urine. |
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| Keywords: | Optical fiber chemiluminescence immunosensor Streptococcus pneumoniae |
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