Abstract: | In previous work, it was found that the bovine serum albumin (BSA) could obviously be damaged by nano-sized TiO2 powder as a sonocatalyst under ultrasonic irradiation. In this work, metronidazole (MTZ) was adopted as a sensitizer to intensify
the damage of BSA molecules. It was found that the damage degree of BSA molecules in the presence of MTZ was more serious
than in the absence of MTZ. That is, under ultrasonic irradiation combined with nano-sized TiO2 powder, the addition of MTZ could remarkably aggravate the damage to BSA molecules. Meanwhile, the damage degree was also
affected by some influence factors, such as ultrasonic irradiation time, ultrasonic irradiation power, MTZ concentration,
solution acidity, ionic strength and solution temperature. In addition, the damage site of BSA molecules was also estimated
by synchronous fluorescence spectra. It was found that the damage site of BSA molecules was mainly at tyrosine (Tyr) residue. |