Simultaneous determination of halogenated benz(a)anthracenes in water by HPLC with fluorometric detection |
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| Authors: | Yasuaki Mori Sumio Goto Sukeo Onodera Syoji Naito Shoji Takitani and Hidetsuru Matsushita |
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| Institution: | (1) Kanagawa Prefectural Public Health Laboratories, 52-2 Nakaocho, Asahi-ku, 241 Yokohama, Japan;(2) Department of Community Environmental Sciences, National Institute of Public Health, 4-6-1 Shirokanedai, Minato-ku, 108 Tokyo, Japan;(3) Faculty of Pharmaceutical Sciences, Science University of Tokyo, 12 Ichigaya-Funagawara, Shinjuku-ku, 162 Tokyo, Japan;(4) Graduate School of Nutritional and Environmental Sciences, University of Shizuoka, 52-1 Yada, 422 Shizuoka, Japan |
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| Abstract: | Summary A HPLC method using a fluorescence detector is described for the determination of halogenated benz(a)anthracenes (BAX, x=Cl or Br) in water. It consists of the following three procedures; (1) liquid-liquid extraction of BAX with benzene, (2) elimination of interfering compounds from the extracts by one dimensional dual-band thin layer chromatography, and (3) quantitative determination of BAX by HPLC equipped with a fluorescence detector. The recoveries of BAX from water samples through the entire analytical procedure amounted to more than 72%. The calibration curves for BAX were linear, e.g., with a range from 1 to 20 ng ml–1 for 7-chlorobenz(a)anthracene (BACl). The lower detection limit for BACl was 20 pg for an injection volume of 20  l. This method has been applied to BAX in river water spiked with benz(a)anthracene B(a)A] at 10 ng ml–1 after chlorination. B(a)A, BACl and BABr were found in the levels of 2.01, 0.16 and 0.13 or less ng ml–1, respectively. |
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