鞘磷脂与胆固醇相互作用的拉曼光谱研究

利用ＦＴ Ｒａｍａｎ光谱研究了不同浓度的胆固醇对鞘磷脂质脂体的作用。结果表明，胆固醇没有改变鞘磷脂的Ｏ Ｃ Ｃ Ｎ＋骨架构象，极性头仍然平行膜表面。Ｃ Ｃ骨架振动强度比Ｉ１０８６／Ｉ１０６４说明，随着胆固醇浓度上升，鞘磷酸分子内部旁式构象也逐渐增多。从Ｉ２８４７／Ｉ２８８０强度比看，胆固醇具有使鞘磷脂脂双层有序性上升，流动性降低的作用，但这种作用随胆固醇浓度上升而逐渐减弱。

FT Raman Studies on the Interaction of Cholesterol with Sphingomyelin

This paper reports the FT Raman spectral study on the interaction of sphingomyelin bilayers with cholesterol of different concentrations. The results show cholesterol do not change the conformation of O C C N + backbone in the sphingomyelin bilayers. The polar head group is still extending parallel to the bilayer surface. On increasing the amount of cholesterol, there is an increase of sphingomyelin inter molecule gauche conformation population, as deduced from changes in the I 1086 /I 1064 ratio. In the presence of cholesterol, the order of the interface increases and the membrane fluidity decreases based on the changes in I 2847 /I 2880 , This interaction, however, decreases with increasing proportion of cholesterol within the bilayers.