Full-field time-resolved fluorescence tomography of small animals |
| |
Authors: | Venugopal Vivek Chen Jin Lesage Frederic Intes Xavier |
| |
Institution: | Department of Biomedical Engineering, Rensselaer Polytechnic Institute, Troy, New York 12180, USA. |
| |
Abstract: | In this experimental investigation, we explore the feasibility of using wide-field illumination for time-resolved fluorescence molecular tomography. The performance of wide-field patterns with a time-resolved imaging platform is investigated in vitro and in a small animal model. A Monte Carlo-based forward model is employed to reconstruct fluorescence yield based on time-gated datasets. An improvement in resolution and quantification when using the time-gate data type compared to the commonly used cw data type is demonstrated in vitro. Furthermore, the feasibility of wide-field strategies for fluorescence preclinical applications is established by an accurate localization of a fluorescent inclusion implanted in the chest cavity of a murine model. |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |
|