| TRANSFER OF FOREIGN GENES BY ELECTROPORATION AND THEIR EXPRESSION IN MAMMALIAN CELLS |
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| 引用本文： | 杨建涛,王秀琴,吴旻. TRANSFER OF FOREIGN GENES BY ELECTROPORATION AND THEIR EXPRESSION IN MAMMALIAN CELLS[J]. 中国科学B辑(英文版), 1989, 0(5) |
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| 作者姓名： | 杨建涛 王秀琴 吴旻 |
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| 作者单位： | Institute of Cancer，Chinese Academy of Medical Sciences，Beijing，Institute of Cancer，Chinese Academy of Medical Sciences，Beijing，Institute of Cancer，Chinese Academy of Medical Sciences，Beijing |
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| 摘 要： | Expression of foreign genes transferred into mammalian cells by electroporation has been studied. The pX1TK gene, pSV2Neo gene and pUCEJ oncogene have been introduced into MLTK-cells and NIH/3T3 cells, respectively. Stable transformation transient expression of TK gene by MLTK-cells as well as stable and malignant transformation of NIH/3T3 cells have been obtained. Transient expression frequency is about 80% and stable transformation frequency is about 10~(-4). Integration of foreign genes into the cellular genome was verified with molecular hybridization. Tumor development was observed after inoculation of transformed celts into nude mice.
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TRANSFER OF FOREIGN GENES BY ELECTROPORATION AND THEIR EXPRESSION IN MAMMALIAN CELLS |
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| YANG JIAN-TAO WANG XIU-QIN WU MIN. TRANSFER OF FOREIGN GENES BY ELECTROPORATION AND THEIR EXPRESSION IN MAMMALIAN CELLS[J]. Science in China(Chemistry), 1989, 0(5) |
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| Authors: | YANG JIAN-TAO WANG XIU-QIN WU MIN |
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| Abstract: | Expression of foreign genes transferred into mammalian cells by electroporation has been studied. The pX1TK gene, pSV2Neo gene and pUCEJ oncogene have been introduced into MLTK-cells and NIH/3T3 cells, respectively. Stable transformation transient expression of TK gene by MLTK-cells as well as stable and malignant transformation of NIH/3T3 cells have been obtained. Transient expression frequency is about 80% and stable transformation frequency is about 10~(-4). Integration of foreign genes into the cellular genome was verified with molecular hybridization. Tumor development was observed after inoculation of transformed celts into nude mice. |
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| Keywords: | gene transfer gene expression oncogene electroporation. |
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