Butanol production using Clostridium beijerinckii BA101 hyper-butanol producing mutant strain and recovery by pervaporation |
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Authors: | Qureshi N Blaschek H P |
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Institution: | (1) University of Illinois, Biotechnology and Bioengineering Group, Department of Food Science and Human Nutrition, 1207 W. Gregory Drive, 61801 Urbana, IL |
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Abstract: | Clostridium beijerinckii BA101 (mutant strain) and C. beijerinckii 8052 (wild type) were compared for substrate and butanol inhibition. The wild-type strain is more strongly inhibited by added
butanol than is the mutant strain. Acetone and butanol were removed from a fed-batch reactor inoculated with C. beijcrinckii BA101 by pervaporation using a silicone membrane. In the batch reactor, C. beijerinckii BA101 produced 25.3 g/L of total solvents, whereas in the fermentation-recovery experiment it produced 165.1 g/L of total
solvents. Solvent productivity increased from 0.35 (batch reactor) to 0.98 g/L·h (fed-batch reactor). The fed-batch reactor
wasfed with 500 g/L of glucose-based P2 medium. Acetone selectivities ranged from 2 to 10 whereas butanol selectivities ranged
from 7 to 19. Total flux varied from 26 to 31 g/m2·h. |
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Keywords: | Clostridium beijcrinckii BA101 acetone butanol fed-batch reactor pervaporation |
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