Differential proteome analysis of colon carcinoma cell line SW480 after reconstitution of the tumour suppressor Smad4 |
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Authors: | Kai Stühler Katharina Köper Kathy Pfeiffer Andreas Tagariello Manfred Souquet Irmgard Schwarte-Waldhoff Stephan A Hahn Wolff Schmiegel Helmut E Meyer |
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Institution: | 1. Medizinisches Proteom-Center, Ruhr-University Bochum, ZKF E 1.43, Universit?tsstra?e 150, 44801, Bochum, Germany 2. Department of Internal Medicine, Knappschaftskrankenhaus, Ruhr-University Bochum, Bochum, Germany 3. Department of Internal Medicine, Knappschaftskrankenhaus, IMBL, Ruhr-University Bochum, Bochum, Germany 4. Department of Gastroenterology and Hepatology, Kliniken Bergmannsheil, Ruhr-University Bochum, Bochum, Germany
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Abstract: | The tumour suppressor gene Smad4 is frequently inactivated in gastrointestinal carcinomas. Smad4 plays a pivotal role in transducing signals of the transforming growth factor-β (TGF-β) superfamily of proteins. Inactivation
of Smad4 seems to occur late during tumour progression when tumours acquire invasive and metastatic properties. Identification of
proteins directly or indirectly regulated by Smad4 would, therefore, ease the future design of new diagnostic and therapeutic strategies for gastrointestinal carcinoma. We
have used human colon carcinoma cell line SW480 stably transfected with Smad4 as an in-vitro model system to identify Smad4-regulated proteins by applying two-dimensional gel electrophoresis (2DE) then MALDI-PMF/PFF-MS. We identified a total of
47 protein species with a Smad4-dependent expression. From the functions of the candidate proteins we obtained new insights into Smad4’s participation in processes, for example apoptosis, differentiation, and proliferation. |
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Keywords: | Differential proteome analysis Two-dimensional gel electrophoresis DPC4 Smad4 Colorectal cancer Tumour suppressor |
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