Amperometric determination of glucose in undiluted food samples |
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| Authors: | A Amine GJ Patriarche G Marrazza M Mascini |
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| Institution: | Free University of Brussels (ULB), Institute of Pharmacy, Campus Plaine, C.P. 205 / 6, Bd du Triomphe, B-1050 Brussels Belgium;Dipartimento Sanita Pubblica, Epidemiologia e Chimica Analitica Ambientale, Sezione Chimica Analitica, Via Gino Capponi 9, 50121 Firenze Italy |
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| Abstract: | Glucose oxidase is immobilized onto a cellulose acetate membrane by glutaraldehyde linkage, and the membrane is used to cover the platinum electrode of a hydrogen peroxide sensor. A silanized polycarbonate membrane then covers the enzyme layer, and extends the linear calibration range to higher concentrations. The sensor, when incorporated into a flow-injection system, allows the determination of glucose at levels up to 1 M in soft drinks at a rate of 60 samples h?1 without sample dilution. |
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| Keywords: | Amperometry Flow system Glucose Foods Soft drinks Enzyme electrodes |
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