Multi-component calibration and analysis in liquid chromatography

Multi-wavelength detectors offer improved detection capabilities for liquid chromatographic methods, but require improvements in data analysis methodology to utilize all the available information. In this work, various methods for improving the quantitative results obtained from liquid chromatography with full spectrum fluorescence detection were studied. The availability of multi-wavelenght information allows overlapped chromatographic peaks to be resolved. Different approaches were investigated for developing calibration models that use all of the available spectral information, and are compatible with a variety of methods for quantification, including factor analysis, Kalman filtering and rank annihilation. These methods were compared for their ability to resolve overlapped chromatographic peaks and their accuracy in quantification.