Flow-injection determination of d-mannitol with immobilized mannitol dehydrogenase |
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| Authors: | Nobutoshi Kiba Yukari Inoue Motohisa Furusawa |
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| Affiliation: | Department of Applied Chemistry and Biotechnology, Faculty of Engineering, Yamanashi University, Kofu 400 Japan |
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| Abstract: | A flow-injection system is described for the determination of d-mannitol. Mannitol dehydrogenase is immobilized on poly(vinyl alcohol) beads and packed in a column (5 cm × 4 mm i.d.). The NADH formed is detected fluorimetrically. The response is linear between 5 × 10?7 and 1 × 10?4 M mannitol and the detection limit is 1 × 10?7 M. The throughput is 30 samples per hour. The reactor is stable for at least 8 weeks. |
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| Keywords: | Flow system Fluorimetry Mannitol |
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